TABLE I.

Primers and Taqman probes that were used in 3C, ChIP, ChIP-3C and RT-PCR assays

Primers for 3C Assays	1	5′ cac gaa tac tct ctg gta att ga 3′
	1′	5′ gca tgg aga caa gat ttc 3′
	2	5′ cta cag cca gca aaa gtc atg g 3′
	3	5′ ggc aac tat tta agg acc c 3′
	4	5′ acc aaa atg tcc acc aac agt 3′
	5	5′ tcc tac ctc ttg aga atg ta 3′
	6	5′ acc atg ttc ctg tgg ctg at 3′
	7	5′ aga agc aga act gtc tag aga ct 3′
	8	5′ cca tcc tat ctt ccc ttc taa gg 3′
	9	5′ cat caa agg tga ggc cat 3′
	10	5′ gga ggg tgg aga aag aat 3′
	11	5′ act gag gca gga agt cct 3′
	12	5′ cct ttg tct tta gtt ctc tgt cta t 3′
	13	5′ cca tca att gtt cat ttc ct 3′
	14	5′ tga ctc tac ttt gtt ccc c 3′
	15	5′ act caa gcc ctg ggt agg ag 3′
	16	5′ ttg taa gcc tct gga gga 3′
	17	5′ ggc acc aac att cat gtg 3′
	AseI PBGD1	5′ cct acc ctg tct aac cta gt 3′
	AseI PBGD2	5′ ttt act att gga gcc atc tg 3′
	BglII PBGD1	5′ gcc taa cta cct ggg gaa tc 3′
	BglII PBGD2	5′ tgt cct gga act ctc tct gt 3′
	Control AseIVκ8f	5′ tct atg cat gga gac aag at 3′
	Control AseIVκ8r	5′ aga gct ttc tta atc cac aa 3′
	ControlAseIVκ4f	5′ gag ata tga tac atg ttc c 3′
	Control AseIVκ4r	5′ cct aca gct tct caa cct gc 3′
	NspI1	5′ gtg aac cat tat gat tgc a 3′
	NspI2	5′ ccc aat gct ttt gca cag tc 3′
	NspI3	5′ gtg gag tag taa ccc acg ga 3′
	18	5′ atg atg atg atg atg atg tt 3′
	25	5′ cag aaa taa gaa act gcc cac gg 3′
	26	5′ cat tat cag ttg acg tgg c 3′
	27	5′ tct gtt gag atg cca act c 3′
	28	5′ gtt tcc atc ttg cta cct c 3′
	29	5′ aaa gaa tac agc atg tga c 3′
	30	5′ caa aat ttg agg tca ttg gg 3′
	31	5′ gga gtc tgc tgt cct tac agg 3′
	32	5′ atg tgc cag agt ggg ttg gta tga 3′
Taqman Probes	AseI Ei	6FAMtga cat gtt cac aat tga gct att act taa TAMRA
	AseI E3′	6FAMcag gcc ttc tgt gca cac aat caa MGB
	AseI Ed	6FAMcac tct gtg tgt att ttt ctt MGB
	BglII Ei	6FAMaag cga cct acc act gtt gcg gtg c TAMRA
	BglII E3′	6FAMatt aaa gca ata cct aca TAMRA
	AseI PBGD	6FAMtcc ctc cc a tgc atg tcc tcc ccg ct TAMRA
	BglII PBGD	6FAMcat gaa ttc aag gct aaa tct ggt TAMRA
Primers for ChIP	1f	5′ cag ctt cct gct aat cag tgc t 3′
	1r	5′ ata cca ccc aac att cag at 3′
	1′f	5′ ctt gct cca cct gcc ttt cag at 3′
	1′r	5′ cac att gtc cag gca ttt gct a 3′
	2f (Eif)	5′ cag agg gga ctt tcc gag agg cc 3′
	2r (Eir)	5′ acc ctg gtc taa tgg ttt gta ac 3′
	3f	5′ cag tcc ttt cta agg ttc acg 3′
	3r	5′ ggt tgc ctt tgc ttc ctg tg 3′
	4f (E3′f)	5′ ata gca act gtc ata gctacc gt 3′
	4r (E3′r)	5′ gca ggt gta tga ggc ttt gga aa 3′
	5f	5′ cta ata gtc cca atg ctc tcc 3′
	5r	5′ tcc ttc ctt tgg tgg ctt c 3′
	6f	5′ ggg tag ttt tcc caa cga aca gt 3′
	6r	5′ gga tgg ttt tct taa aga gtg tga g 3′
	7f (Edf)	5′ ggg aca gat cac cat ttc gtt aa 3′
	7r (Edr)	5′ ctt cag gcc agc tgg gca gtg ag 3′
	β-actinf	5′ cgc cat gga tga cga tat cg 3′
	β-actinr	5′ cga agc cgg ctt tgc aca tg 3′
	α-actinf	5′ acg gac gta agc ctc act tc 3′
	α-actinr	5′ tac ctg ctg ctc tga ctc tg 3′
Primers for RT-PCR	Vκ8f	5′ ggt acc tgt ggg gac att gtg 3′
	Cκr	5′ gag gca cct cca gat gtt aac 3′
	Vκ4f	5′ aca atg act tgc agg gcc 3′
	5′ germline transf	5′ cca cat gcc ttt ctt cag gga caa gtg gga 3′
	5′ germline transr	5′ gtt atg tcg ttc ata ctc gtc ctt ggt caa c 3′
	PBGDf	5′ tgt ccc ggt aac ggc ggc gcg gcc aca ac 3′
	PBGDr	5′ gcc acc aca gtc tcg gtc tgt atg cga gc 3′