Vero cells were mock-transfected or transfected with siRNA to Cog3. 72 hours after transfection, the cells were treated with 1 µg/ml SubAB HF555 for one hour. Control (A) and Cog3 cells (B) were fixed in 4% paraformaldehyde, permeabilized with Triton X-100, stained with anti-TGN46, anti-EEA1, anti-Rab7, and anti-LAMP2, and developed using HiLyte-488-conjugated-anti-mouse and HiLyte-488-conjugated-anti-rabbit antibodies. Samples were viewed by dual-laser confocal microscopy (63X oil objective). Bar, 10 µm. Arrows demonstrate colocalization between TGN46 and SubAB HF555.