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. Author manuscript; available in PMC: 2010 Sep 4.
Published in final edited form as: Cell Stem Cell. 2009 Aug 6;5(3):267–278. doi: 10.1016/j.stem.2009.06.004

Figure 2.

Figure 2

Expression profiling at 2-hair cycle stages. (A) Cell subpopulations sorted and profiled by expression microarrays. (B) Gene (top), and cell population (left) dendogram heat map shows 2 main population clusters (blue star). Newly divided CD34+/α6+ cells (Bu 1–2 Div) cluster with a different group at each stage. (C) Genes changed in Bu (1–2 Div) cell populations relative to Bu (0 Div) are shown as percent of 4 hair lineages gene signatures: CD34+/α6+ bulge (Bu). CD34−/α6+ non-bulge (NonBu), matrix (Mx), dermal papillae (DP) (Table S2). Note down-regulation of bulge signature genes and higher up-regulation of Mx genes at PD18-PD21. DP was a negative control as an unrelated lineage and showed low and similar representation in both populations. (D). QRT-PCR (relative to GAPDH) of several Non-Bu or Matrix signature genes found up-regulated in our microarray in Bu (1–2 Div) relative to Bu (0 Div) shown with SEM. N=2 mice per stage (triplicate wells). Regular RT-PCR performed for 4 additional mice per stage is not shown. (E) Same QRT-PCR analysis for LGR5; a second experiment with another set of mice at each stage is shown in Fig. S2.