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. Author manuscript; available in PMC: 2010 Sep 4.
Published in final edited form as: Cell Stem Cell. 2009 Aug 6;5(3):267–278. doi: 10.1016/j.stem.2009.06.004

Figure 5.

Figure 5

Multipotency of bulge cells in vivo.

(A) Hair bulb with indicated differentiated lineages. Outer root sheath (ORS); IRS (inner root sheath made of cuticle (C), Huxley (Hu), and Henle (He) cell layers); hair shaft “core” made of 3 cell layers: (Cu) cuticle, (Co) cortex, (Me) medulla. Cp is ORS companion layer, included with the IRS category for simplification. (B) Quantification of PD35 labelling patterns in mice injected with tamoxifen at PD17. Lineages containing X-Gal+ cells are indicated at bottom, shown with SEM in 3 mice (>60 labelled HFs/mouse). (C&D) X-Gal bulb-only patterns in thick (90µm) or thin (20µm) serial sections. Scale bar, 50µm. (E) Cross-sectional images of X-gal stained skin from PD17-PD35 chase 20µm shows multiple differentiated layers in a majority of HFs. Scale bar, 10 µm. (F) Patterns of labelling, with SEM shown as % of a defined pattern from total labelled HFs (N= 3 mice, > 200 labelled HFs/mouse).