Figure 4.

Promoter methylation and gene expression of EPHA7 in human lymphomas. (a) Methyl-specific PCR was performed on Nalm-6 or 293T cell lines, a pool of 5 non-malignant hyperplastic human lymph nodes (P), reactive tonsil (T) and primary human GC-NHL samples including Burkitt lymphomas (BL), DLBCL, follicular lymphomas (FL) and MZL. Upper and lower bands correspond to PCR products amplified by primers specific to unmethylated (U) or methylated (M) CpG sites following bisulfite conversion. (b) EPHA7 expression was measured by real-time PCR for the above samples, including each individual hyperplastic lymph node (N1–N5) from the pooled lymph nodes. (c) Real-time PCR analysis of EPHA7 from Nalm-6 and Ramos before or after treatment with 5-aza-2′-deoxycytidine (Aza) and/or 100 nM TSA.