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. Author manuscript; available in PMC: 2009 Oct 5.
Published in final edited form as: Oncogene. 2007 Jan 29;26(29):4243–4252. doi: 10.1038/sj.onc.1210211

Figure 5.

Figure 5

A variant transcript of EPHA7 encodes EphA7-S, a soluble protein secreted by lymphocytes. (a) Nucleotide sequence and predicted amino acid sequence for full-length EphA7 (EphA7-FL) at the end of exon 5 (arrow-splice site) and for the variant transcript (EphA7-S) based on sequencing of a cDNA clone from murine splenic B-cells. (b) EphA7 expression was determined by Western blot (polyclonal antibody) on WCL or serum-free CM from the human B-cell line BJAB, sorted normal human tonsil B or T cells, normal murine whole splenocytes and sorted normal murine splenic B or T cells. WCL from Nalm-6 cells stably transfected with EphA7-FL served as a positive control (+). Arrow indicates EphA7-S (E7-S), asterisk indicates unidentified immunoreactive band in BJAB CM. (c) Western blots on CM from sorted normal human tonsil B or T cells using rabbit polyclonal (PAb) or rat monoclonal (MAb) antibodies against the extracellular domain of EphA7.

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