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. Author manuscript; available in PMC: 2010 Sep 10.
Published in final edited form as: J Med Chem. 2009 Sep 10;52(17):5356–5364. doi: 10.1021/jm801176e

Figure 3.

Figure 3

Cell-based assay comparing the effects of D-carba T on the replication of an HIV-1 vector containing wt HIV-1 RT and vectors that replicate using NRTI-resistant HIV-1 RTs. AZT-R and SSGR+T215Y are efficient at ATP-dependent pyrophosphorolysis (excision), while M184V interferes with the incorporation of NRTI-TPs. The vectors also express luciferase from the nef reading frame. A) HOS cells were infected with the various HIV-1 vectors in the presence of increasing levels of D-carba T. Luciferase activity in the absence of D-carba T was considered to be 100 and the luciferase activity in the presence of the different concentrations of D-carba T was normalized to this value to give relative infectivity. B) Assay done in HOS 313 cells (which express HSV-TK). C) HOS cells were infected with the various HIV-1 vectors in the presence of increasing levels of AZT.