TABLE 2.
Plasmidb | Strain | No. of transformants
|
Silencing frequency (%) | ||
---|---|---|---|---|---|
Albino | Wild type | Total | |||
pMAT1253 (hpRNA) | wtc | 543 | 96 | 639 | 85.1 |
dcl-1− | 544 | 82 | 626 | 86.9 | |
pMAT647 (s-transgene) | wt | 26 | 489 | 515 | 5.0 |
dcl-1− | 93 | 1,513 | 1,606 | 5.7 |
The colors of the transformants were observed after 48 h under illumination with white light.
hpRNA, construct expressing hpRNA; s-transgene, construct expressing the sense transgene.
wt, wild type.