TABLE 3.

Gene silencing by self-replicative sense and inverted-repeat transgenes in the dcl-2⁻ mutant MU410 and a MU410 transformant carrying a wild-type allele of dcl-2^a

Plasmidb	Strain	No. of transformants			Silencing frequency (%)
		Albino	Wild type	Total
pMAT1253 (hpRNA)	wtc	275	49	324	84.8
	dcl-2−	15	500	515	3.47
	dcl-2−dcl-2+	270	90	380	71.8
pMAT647 (s-transgene)	wt	25	82	107	23.4
	dcl-2−	0	129	129	0
pLEU (control)	wt	0	340	340	0
	dcl-2−	0	130	130	0
	dcl-2−dcl-2+	0	50	50	0

^aThe colors of the transformants were observed after 48 h under illumination with white light.

^bhpRNA, construct expressing hpRNA; s-transgene, construct expressing the sense transgene.

^cwt, wild type.