FIG. 2.

Southern hybridization of pmt deletion strains. Genomic DNAs of the wild type (A850) (WT) and strains transformed with deletion cassettes for pmtA (ATK08) (A), pmtB (ATK16) (B), and pmtC (ATK38) (C) were digested with the restriction enzymes indicated and probed with a radiolabeled upstream fragment of the corresponding pmt. B, BglII; C, ClaI; E, EcoR I; H, HindIII; N, NcoI; X, XbaI. The approximately 7-kb band in ΔpmtB cut with XbaI is from the left flank of the probe. This band is obscured by the 6.97-kB band in the wild type.