TABLE 1.
A. nidulans strains and plasmids
| Strain or plasmid | Genotype/phenotype | Source or reference |
|---|---|---|
| Strains | ||
| A850 | argB2::trpC_B methG | FGSC |
| A773 | pyrG89 wA3 pyroA4 | FGSC |
| TNO2A7/A1145 | pyrG89 ΔnkuA::argB pyroA4 riboB2 | 21a |
| ATK08 | pyrG89 argB2::trpC_B pyroA4 ΔpmtA::AfpyrG | This study |
| ATK16 | pyrG89 ΔpmtB::AfpyrG argB2 pyroA4 | This study |
| ATK38 | pyrG89 wA3 argB2 pyroA4 ΔpmtC::AfpyrG | This study |
| ATK40 | pyrG89 wA3 argB2::trpC_B pyroA4 | This study |
| ATK45 | pyrG89 argB2::trpC_B nkuA::Afpyro pyroA4 | This study |
| ATK57 | pryG89 ΔpmtB::Afpyro nkuA::argB pyroA4 riboB2 | This study |
| ATK88 | pyrG89 wA3 argB2::trpC_B pyroA4 ΔpmtC::AfargB | This study |
| ATK94 | pyrG89 argB2::trpC_B ΔpmtB::AfpyrG pyroA4 ΔpmtA::Afpyro | This study |
| ATK104 | pryG89 wA3 ΔpmtB::Afpyro argB2 pyroA4 | This study |
| ATK147 | A850::AfargB-gpd(P)-AN5660-S-tag | This study |
| ATK148 | ATK16::AfargB-gpd(P)-AN5660-S-tag | This study |
| ATK149 | ATK08::AfargB-gpd(P)-AN5660-S-tag | This study |
| ATK160 | ATK38::AfargB-gpd(P)-AN5660-S-tag | This study |
| Plasmids | ||
| pDV2 | AmprargB-gpd(p)-ccdB-sgfp | 32a |
| pFNO3 | Kanr GA5-GFP AfpyrG | 36 |
| pHL85 | Ampr Kanr GA5-mCherry Afpyro | B. R. Oakley |
| pAO81 | GA4-S-Tag AfpyrG | 36 |
| pAfargB2 | AmprargB2 | G. S. May |
| pTK59 | pENTR/D-TOPO::AN5660-S-tag | This study |
| pTK64 | pDV2::AN5660-S-tag | This study |