FIG. 7.

Comparison of DCG secretion from wild-type and ΔGRT1 ΔGRT2 cells. Stationary overnight cultures (50 ml) of wild-type (WT) and ΔGRT1 ΔGRT2 (ΔGRT1-2; line A) cells were pelleted, resulting in similarly sized cell pellets (left panel). Cells were resuspended, stimulated with dibucaine for 20 s, and recentrifuged to produce a pellet of cells with an overlying flocculent (right panel). The flocculent corresponds to the DCG contents that were released from the cells. The boundary between the pelleted cells and the flocculent is indicated by arrows. The postdibucaine cell pellet from ΔGRT1 ΔGRT2 cultures is larger because a smaller proportion of cells is trapped in the ΔGRT1 ΔGRT2 flocculent (right panel).