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. 2009 Aug 17;29(20):5590–5603. doi: 10.1128/MCB.00039-09

FIG. 7.

FIG. 7.

An Su(s) target site is found within the first 267 nt of αβ sequences. (A) Map of an αγ element, which consists of Hsp70 promoter and 5′ UTR sequences joined to αβ sequences. Four fragments (αγ A to D) were cloned into a LacZ reporter vector and transformed into flies. The numbers at the boundaries of the cloned fragments are relative to the Hsp70 transcription start site. pA, polyadenylation signal. (B) Representative Northern blots of RNA from two transformant lines after heat shock induction of the transgenes. Probes are indicated on the right. RT, control. (C) Graph illustrating the results of the Northern analysis of two heat shock-inducible LacZ reporter transgenes lacking αβ sequences. The RNAs produced by these transgenes are not regulated by Su(s). (D) Graph illustrating the results of the Northern blot analysis of experiments with three heat shock-inducible LacZ reporter transgenes containing variable amounts of αβ sequences. Two different transformant lines containing the αγ-B and αγ-C constructs were analyzed. Su(s) downregulates the RNAs produced by these transgenes at 32°C. The results shown in panels C and D are from two different experiments. LacZ RNA levels were normalized with rp49.