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. 2009 Aug 17;29(20):5505–5516. doi: 10.1128/MCB.00388-09

FIG. 3.

FIG. 3.

Autoubiquitination of the recombinant RING domains of Pex2, Pex10, and Pex12. Isolated GST fusions of the RING finger domains of the three RING peroxins, Pex2 (aa 215 to 271), Pex10 (aa 238 to 337), and Pex12 (aa 293 to 399), were incubated with the indicated E2 enzymes in the presence or absence of E1, Ub, and methylated Ub (meUb) (A) or with Ub in the presence or absence of E1 (B). Autoubiquitination of the proteins was monitored by immunoblot analysis with antibodies against GST (A) or Ub (B). Ubc4 and Pex4 but not Ubc7 facilitated the modifications of the three RING domains.