TABLE 1.
Oligonucleotides used in this study
| Purpose and name | Protein or other element(s) (strand) | Plasmid or DNA | Sequence |
|---|---|---|---|
| Integration | |||
| PEX2 | ProtA (sense) | pProtA/HU | 5′ GATGCCTGTGGATCCTCTGGGAGACTGACCGCCTCACCAGTGTACGGTGAAGCTCAAAAACTTATT 3′ |
| ProtA (antisense) | pProtA/HU | 5′ ATAGTATACACATATATAGAGATACAAGCGAGGGAACGGGGCCCATTTTACTTATAATACAGTTTTTTAG 3′ | |
| PEX10 | ProtA (sense) | pProtA/HU | 5′ GCAGACAACACTGTCAACCACAGGAAATTCTGGTCCTGCGGCAAGGTGAAGCTCAAAAACTTATT 3′ |
| ProtA (antisense) | pProtA/HU | 5′ GGCCTGTGGACAATGCTAAAAGAGTAGTCAAATTATTGATTATTTACTTATAATACAGTTTTTTAG 3′ | |
| PEX12 | ProtA (sense) | pProtA/HU | 5′ ACATACAACAAACACACGAATAAATGGGAAGTTGTGACAGGTATTAGGAAGCTACTAATCGGTGAAGCTCAAAAACTTATT 3′ |
| ProtA (antisense) | pProtA/HU | 5′ TGCTTGTAACACAACTGCAAAGAGGTGGAACAAGTGACATGAATTACCTGCTGGATAACATTTACTTATAATACAGTTTTTTAG 3′ | |
| PEX2 1 to 221 | ProtA (sense) | pProtA/HU | 5′ AAAAAACCACCAAGGTCTCGTTCTACGACTACTTATAAGACAGTTGGTGAAGCTCAAAAACTTATT 3′ |
| ProtA (antisense) | pProtA/HU | 5′ AGCGATCTGGTATGGGTTGGTTGGAAAACCGCCGCAACGGGGGCAATTTTACTTATAATACAGTTTTTTAG 3′ | |
| PEX10 1 to 238 | ProtA (sense) | pProtA/HU | 5′ TCTACGCTCGGGTCCTGGATTTATGGGAGAAAAAGGACCAATGATGGTGAAGCTCAAAAACTTATT 3′ |
| ProtA (antisense) | pProtA/HU | 5′ ATGTTCTGAACGCTCCTGTAGACCAACAGACGACCTAGTAATTGAATTTTACTTATAATACAGTTTTTTAG 3′ | |
| PEX12 1 to 333 | ProtA (sense) | pProtA/HU | 5′ TCACATAGTGACAAAACGGAAGATAAAGAGGGAGTTTCTGAGGCCGGTGAAGCTCAAAAACTTATT3′ |
| ProtA (antisense) | pProtA/HU | 5′ TTCCAATACACAGGGGTTTTGTACAGTTTTTTCGCAAACGGGACAATTTTACTTATAATACAGTTTTTTAG 3′ | |
| PEX2 | TEV-ProtA (sense) | pYM8 | 5′ GATGCCTGTGGATCCTCTGGGAGACTGACCGCCTCACCAGTGTACCGTACGCTGCAGGTCGAC 3′ |
| ProtA (antisense) | pYM8 | 5′ ATAGTATACACATATATAGAGATACAAGCGAGGGAACGGGGCCCTATCGATGAATTCGAGCTCG 3′ | |
| PEX4 | HA (sense) | pYM3 | 5′ AAAGTACTTCCTAGCAGAAAGAGAGCGGATC 3′ |
| HA (antisense) | pYM3 | 5′ CCCATTGTTTGCCATTCGAACACATCCATCC 3′ | |
| Cloning | |||
| UBC7 | ORFa plus EcoRI (sense) | Genomic DNA | 5′ GAATTCATGTCGAAAACCGCTCAG 3′ |
| ORF plus NotI (antisense) | Genomic DNA | 5′ GCGGCCGCTCAGAATCCTAATGATTT 3′ |
a
ORF, open reading frame.