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. 2009 Aug 10;29(20):5578–5589. doi: 10.1128/MCB.00283-09

FIG. 5.

FIG. 5.

TCR and integrin-initiated SLP-76 microclusters exhibit different dynamic properties. (A) J14:GFP-SLP-76 cells were deposited onto TCR and integrin (FN) stimulatory surfaces and imaged over time by TIRF microscopy, and snapshots from indicated time points (indicated in seconds) are shown. Bars, 5 μm. (B) GFP-SLP-76 clusters were tracked at all time points, and trajectories are shown overlaid on the sample cell. (C) (Left panel) The displacement of GFP-SLP-76 clusters was calculated, and the results are shown with respect to elapsed time, with TCR results represented in black and integrin (FN) results in gray. (Right panel) Numbers of GFP-SLP-76 clusters in representative cells were counted at multiple time points, and percentages representing the maximum numbers of clusters counted at any time point are shown. Data represent means ± standard deviations of the results of 10 TCR and 4 FN experiments.