FIG. 1.
Two functional NES identified in menin. (A) Potential NES sequences of menin (NES33-41, NES253-259, and NES258-267) and HIV Rev (REV74-83) sequences were inserted into pEGFP-C1 vectors to generate fusion proteins. (B) Three potential NES in menin (aa 36 to 41, 254 to 259, and 258-267) were identified, inserted into the pEGFP-C1 vector, and transfected into Men1−/− MEFs. The GFP fluorescence (green) was observed at 24 h after transfection. A HIV Rev NES was used as a positive control. NES1 (aa 36 to 41) and NES2 (aa 258 to 267) were functional in export from the nucleus. (C) Sequence conservation of menin NES. NES1 (aa 36 to 41) is located in the N terminus and NES2 (aa 258 to 267) in the middle of menin. NLS1, NLS2, and NLSa are located in the C terminus of menin, as reported previously (20, 45). Both NES1 and NES2 are highly conserved in human, mouse, rat, and zebrafish.