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. Author manuscript; available in PMC: 2010 Jun 30.

Published in final edited form as: Biochemistry. 2009 Jun 30;48(25):5839–5848. doi: 10.1021/bi802309y

TABLE 3.

Summary of kinetic constants.

	CO
Protein	k_on (µM⁻¹s⁻¹)	k_off (s⁻¹)	K_d (µM)
Y171FdevS	0.73 ± 0.071^b	2.43 ± 0.15^b	3.33
DevS	0.31 ± 0.031^b	2.57 ± 0.40^b	8.29
DevSGAFA/B	0.26 ± 0.024^b	2.45 ± 0.26	9.42
DevSGAFA	0.18 ± 0.007^b	2.74 ± 0.40	15.22
EcDosH(32)	0.0011	0.011	10
AxPDEAH(31)	0.21	0.058	0.28
BjFixL(30)	0.005	0.045	9
RmFixLT(30)	0.012
RmFixLH(30)	0.017	0.083	4.9
	O₂
Protein	k_on (µM⁻¹s⁻¹)	k_off (s⁻¹)	Kd (µM)
Y171FDevS	32.4^a	3.85 ± 0.69^b	0.12
DevS	11.8^a	6.79 ± 0.33^b	0.58
EcDosH(32)	0.0026	0.034	13
AxPDEAH(31)	6.6	77	12
BjFixL(30)	0.145	20	138
RmFixLT(30)	0.217	11	51
RmFixLH(30)	0.217	6.8	31

^a

The oxygen association constants were determined in this study using laser flash photolysis.

^b

All other rates were determined in this work using the stopped flow method.