TABLE 6.
Autooxidation rates of various heme proteins, where Mb is myoglobin; Hb is hemoglobin; EcDos is the Escherichia coli Dos. Conditions:
| Protein | k (h−1) | t1/2 (h) |
|---|---|---|
| EcDos-PAS(47)a | 0.3 | 2.3 |
| EcDos-PAS R97A(47) a | 570 | 0.0012 |
| EcDos-PAS R97E(47) a | 2760 | 0.00025 |
| EcDos(48) | 0.7 | 1 |
| AxPDEA1(31)b | >12 | |
| BjFixL(30) c | 2.8 | 0.25 |
| RmFixLT(30) c | 1.9 | 0.37 |
| RmFixLH(30) c | 2.3 | 0.3 |
| SWMb(49) c | 0.06 | 12 |
| SWMb H(E7)L(49) c | 10.4 | 0.07 |
| Bovine Mb(50) d | 0.01 | 63 |
| Human Hb chain alpha(51) e | 0.05 | 13 |
| human Hb chain beta(51) e | 0.09 | 7.8 |
a)
pH 8, 25 °C;
b)
pH 8, Tris buffer, 23 °C;
c)
pH7, 37 °C;
d)
pH 9, 25 °C;
e)
pH 8, 35 °C.