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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Menopause. 2009 Jul–Aug;16(4):748–755. doi: 10.1097/gme.0b013e3181a4c76a

FIG. 3.

FIG. 3

The ER antagonist ICI 182780 specifically blocks the induction of luciferase activity by PD through ERα in MCF-7 cells transiently transfected with a pERE-luciferase plasmid. Cells were treated with 1 nM E2 or 1 nM E2 + 10 nM ICI 182780 or 20 μg/ml PD or 20 μg/ml PD + 10 nM ICI 182780 to assess induction. Results are expressed as fold induction above control (vehicle solvent). Abbreviations: E2 = 17β-estradiol; PD = Pimenta dioica; ICI = ICI182780. Data represent the mean ± SD of three separate experiments performed in triplicate. * P < 0.001 versus control solvent (none) or **p < 0.05 versus E2.