Figure 3.
NG protects HaCaT cells from UVB-induced apoptosis. Exponentially growing HaCaT cells were washed with PBS, irradiated with 15 or 30 mJ cm−2 of UVB, and then incubated for 6 h in culture medium with or without 5 or 10 µm NG. (A) Agarose gel analysis of fragmentation patterns of cellular DNA isolated from treated or untreated cells. (B) The percentage of cell populations at sub-G0/G1 (sub-G1) phase as measured by flow cytometry. *Shows significant difference from UV-treated cells at P < 0.05.