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. Author manuscript; available in PMC: 2009 Oct 5.
Published in final edited form as: Brain Res. 2008 Jan 3;1197:13–22. doi: 10.1016/j.brainres.2007.12.058

Figure 7. Presence of NMMHC II-a within the protein homogenate from mitochondrial fractions.

Figure 7

Immunoblot analysis of protein homogenate from the whole cell extract (WCE) and mitochondrial fractions (MF) isolated from the cochlea and kidney demonstrates presence of 220 kDa NMHC-IIa. The mitochondrial fractions from the cochlea and kidney are positive for presence of the mitochondrial marker Cytochrome C (15 kDa) and negative for cytoplasmic marker GAPDH (37 kDa). The protein homogenate from the whole cell extracts (WCE) from which the mitochondria were fractionated is immuno-positive for NMHC-IIa, GAPDH and Cytochrome C.