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. Author manuscript; available in PMC: 2009 Dec 5.
Published in final edited form as: Circ Res. 2008 Oct 23;103(12):1393–1401. doi: 10.1161/CIRCRESAHA.108.176768

Figure 5. PDGFRβ stimulation induces epicardial cell migration.

Figure 5

(A) Quantification of WT1+ cells in right and left lateral ventricles of E15.5 hearts from the indicated genotypes. Numbers in parentheses indicate the number of independent hearts quantified. All mutant alleles exhibited a reduction in WT1+ cells within the myocardium compared to those present in wild type myocardium. (One- way ANOVA, *p<0.001). See text for allele description. (B-E) Migration of epicardial cells by CCFSE dye tracing. Inset in B shows a time zero E13.5 heart labeled with CCFSE. Arrowheads mark the epicardial boundary. F. Quantification of adeno-viral GFP-labeled epicardial cells that have migrated into the myocardium. Numbers in parentheses indicate the number of hearts assayed. LY=LY294002. All samples were compared to wild type cell migration. (Two-way ANOVA, *p<0.001)