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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Cell Death Differ. 2009 Mar 20;16(6):899–909. doi: 10.1038/cdd.2009.22

Figure 1.

Figure 1

3-NP induces punctate mitochondrial morphology. DsRed2-Mito expressing purified cortical neurons were exposed to 3-NP (10 mM) at 12 DIV and changes in mitochondrial morphology were visualized using wide-field fluorescence 3D time-lapse imaging. (A) Representative frames of the time-lapse series of a control neuron (upper panel) and 3-NP-exposed neuron (lower panel) at time zero and after 6 h. Mitochondrial morphology of the control neuron remains unaltered during this time period (upper panel). In contrast, the 3-NP-exposed neuron (lower panel) exhibits a change from tubular to punctate mitochondrial morphology. Scale bar, 10 μm. (B) Representative frames of a 3D time-lapse series featuring a DsRed2-Mito expressing cortical neuron at 300 min, 375 min and 510 min after 3-NP exposure. (a and b, lower panels) show a 2.5× zoom of the region of interest (ROI), marked within the white square. Mitochondria show both condensation (a, white arrowheads) as well as fragmentation (b, white arrows). Scale bar, 10 μm