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. Author manuscript; available in PMC: 2009 Oct 5.
Published in final edited form as: Nat Struct Mol Biol. 2009 Feb 8;16(3):325–333. doi: 10.1038/nsmb.1555

Figure 3.

Figure 3

Effects of locking the registry of the stalk coiled coil on ATP-sensitive MT-binding activity. HFG380 (WT) and its double-Cys mutants were untreated or oxidized, and then their MT-binding activities were measured by a cosedimentation assay with various concentrations of MT in the presence or absence of 3.3 mM ATP. Data were obtained using two independent dynein preparations. The smooth curves are the best fits of the data to hyperbolas with dissociation constant (Kd) and maximum binding (Bmax) values given in Table 1.