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. Author manuscript; available in PMC: 2009 Oct 5.
Published in final edited form as: Nat Cell Biol. 2008 Feb 10;10(3):272–282. doi: 10.1038/ncb1689

Figure 2. The lumenal domain of SEL1L scaffolds Hrd1, OS-9 and XTP3-B.

Figure 2

a. Schematic diagram of full length and truncated SEL1L. Potential N-linked glycosylation sites (Ψ) and Sel-1 domains (triangle) are indicated. b. S-tagged versions of full length (WT), truncated (1-372, 1-737) and KDEL-amended (1-372KDEL, 1-737KDEL) SEL1L expressed in HEK293 cells. From normalized protein amounts, coprecipitation of indicated proteins (OS-9, XTP3-B and Hrd1) with representative input controls (20% of starting crude lysate) were assessed by immunoblot with the designated antibodies. c. Coexpression of S-SEL1LWT with shRNA targeting the indicated OS-9 isoforms and affinity purification as in Fig. 2b. Immunoblots were probed for S-tag and OS-9. shRNA specificity is also presented in Supplemental Information (Fig. S2, S3) d. Pulldowns of SEL1LWT treated -/+ EndoH and probed for OS-9.