Figure 2. RvE1 and PD1 increase macrophage phagocytic activity in vivo and in vitro.

(A) In vivo phagocytosis with representative dot plots of FACS analysis. (B) (Top) Time course of eicosanoid generation and phagocytosis activity in vitro. Results are the mean ± s.e.m. (n=3). LXA₄ amounts are expressed as pg/coincubation (0.4x10⁶ PMN+0.2x10⁶ macrophages in 0.2 ml). *p=0.01, **p=0.02 (vs. time 0 or 120 min). Phagocytosis activities are expressed as [F4/80⁺Gr-1⁺/F4/80⁺]x100%. ^#p=0.03, ^##p=0.01 (vs. time 120 min). (Inset) LXA₄ and LTB₄ generation at 60 min. (Bottom left) MS/MS spectrum of LXA₄. (Bottom right) RvE1 generation. Macrophages were incubated with ASA (500 μM, 30 min) followed by EPA (20 μM, 45 min) prior to incubation with apoptotic PMN. RvE1 was determined by LC/MS/MS and expressed as pg/coincubation (2x10⁶ PMN+1x10⁶ macrophages in 1 ml). (C) Phagocytosis of apoptotic PMN in vitro. Results are the mean ± s.e.m. (n=3-4) and expressed as percent increase of F4/80⁺Gr-1⁺ macrophages compared to vehicle alone. Cytokine/chemokine levels are the mean ± s.e.m. (n=3). *p<0.05, **p<0.01, ***p<0.001, compared to vehicle alone. (D) Phagocytosis of zymosan and latex particles in vitro. Results are the mean ± s.e.m. (n=3) and expressed as percent increase of F4/80⁺zymosan⁺ or F4/80⁺latex⁺ macrophages compared to vehicle alone. Results are the mean ± s.e.m. (n=3). *p<0.05, **p<0.01 compared to vehicle alone.