Table 1.
Primary Antibody combination | Primary Antibody concentration | Supplier | Secondary Antibodies | Sequential Immuno-reaction | Secondary Antibodies | |
---|---|---|---|---|---|---|
CAT | gp VGLUT1 | 1:5000 | Chemicon, Harlow, UK | Cy-5 | ||
rbt VGLUT2 | 1:5000 | Chemicon, Harlow, UK | Alexa488 | |||
| ||||||
RAT | mo CTb | 1:250 | A. Wikström, University of Gothenburg | Rh Red | ||
A | gt VAChT | 1:5000 | Millipore, USA | Alexa488 | ||
gp VGLUT1 | 1:5000 | Cy-5 | ||||
| ||||||
B | mo CTb | 1:250 | Rh Red | |||
gt VAChT | 1:5000 | Alexa488 | ||||
gp VGLUT2 | 1:5000 | Chemicon, Harlow, UK | Cy-5 | |||
| ||||||
C | mo CTb | 1:250 | Rh Red | |||
gt VAChT | 1:5000 | Alexa488 | ||||
gp VGLUT3 | 1:5000 | Chemicon, Harlow, UK | Cy-5 | |||
| ||||||
D | shp NOS | 1:2000 | P. Emson, University of Cambridge | Rh Red | ||
gp VAChT | 1:5000 | Millipore, USA | Alexa488 | |||
rbt VGLUT2 | 1:5000 | Chemicon, Harlow, UK | Cy-5 | |||
| ||||||
E | mo CTb | 1:250 | Rh Red | |||
gp VGLUT1 | 1:5000 | Alexa488 | ||||
rbt CB | 1:2000 | Swant, Bellizona, Switzerland | Cy-5 | |||
| ||||||
F | mo CTb | 1:250 | Rh Red | |||
gp VGLUT2 | 1:5000 | Alexa488 | gt VAChT | Cy-5 | ||
rbt CB | 1:2000 | Cy-5 | ||||
| ||||||
G | mo CTb | 1:250 | Rh Red | |||
gt VAChT | 1:5000 | Alexa488 | gp VGLUT1 | Cy-5 | ||
rbt CB | 1:2000 | Cy-5 |