Table 1.

Summary of primary and secondary antibody combinations and concentrations used in the current study. All secondary antibodies were raised in donkey and conjugated to Rhodamine Red (Rh Red; 1:100), Cyanine 5.18 (Cy-5;1:100; both supplied by Jackson Immunoresearch, Luton, UK) and Alexa-fluor 488 (Alexa488; 1:500; Molecular Probes, Eugene, Oregon, USA). CB; calbindin, gp, guinea pig; gt, goat; mo, mouse; rbt, rabbit; shp, sheep.

	Primary Antibody combination	Primary Antibody concentration	Supplier	Secondary Antibodies	Sequential Immuno-reaction	Secondary Antibodies
CAT	gp VGLUT1	1:5000	Chemicon, Harlow, UK	Cy-5
	rbt VGLUT2	1:5000	Chemicon, Harlow, UK	Alexa488
RAT	mo CTb	1:250	A. Wikström, University of Gothenburg	Rh Red
A	gt VAChT	1:5000	Millipore, USA	Alexa488
	gp VGLUT1	1:5000		Cy-5
B	mo CTb	1:250		Rh Red
	gt VAChT	1:5000		Alexa488
	gp VGLUT2	1:5000	Chemicon, Harlow, UK	Cy-5
C	mo CTb	1:250		Rh Red
	gt VAChT	1:5000		Alexa488
	gp VGLUT3	1:5000	Chemicon, Harlow, UK	Cy-5
D	shp NOS	1:2000	P. Emson, University of Cambridge	Rh Red
	gp VAChT	1:5000	Millipore, USA	Alexa488
	rbt VGLUT2	1:5000	Chemicon, Harlow, UK	Cy-5
E	mo CTb	1:250		Rh Red
	gp VGLUT1	1:5000		Alexa488
	rbt CB	1:2000	Swant, Bellizona, Switzerland	Cy-5
F	mo CTb	1:250		Rh Red
	gp VGLUT2	1:5000		Alexa488	gt VAChT	Cy-5
	rbt CB	1:2000		Cy-5
G	mo CTb	1:250		Rh Red
	gt VAChT	1:5000		Alexa488	gp VGLUT1	Cy-5
	rbt CB	1:2000		Cy-5