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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Nat Cell Biol. 2009 Apr 19;11(5):637–643. doi: 10.1038/ncb1870

Figure 5.

Figure 5

Alk3a and Alk8 associate in a complex only when Bmp2b and Bmp7 are present. a: Anti-FLAG IP and anti-HA IB from lysates of WT embryos expressing Alk3a-HA and Alk8-FLAG treated with or without crosslinker (lanes 1–4), or expressing Alk4-HA with either Alk3a-FLAG or Alk8-FLAG (lanes 5–10). b: Anti-FLAG IP and anti-HA IB from lysates of crosslinked WT, bmp7 protein null mutant, or bmp2b MO treated embryos expressing Alk8-HA and Alk3a-FLAG. Full blots are shown in Supplementary Information, Figure S6. c: Predicted arrangement of a Bmp2-Bmp7 heterodimer with Alk3/6 and Alk2/8. α: alpha helix, β: beta strands of ligand monomers. d: Model of heterodimer-mediated DV patterning.