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. Author manuscript; available in PMC: 2010 Feb 15.
Published in final edited form as: J Immunol. 2009 Feb 15;182(4):1940–1953. doi: 10.4049/jimmunol.0800443

Figure 5. L5178Y lymphoma cells have unique immunophenotype that can be altered by exposure to Mϕ.

Figure 5

A, L5178Y and EL4 T-cell lymphoma cell lines, A20 B-cell lymphoma cell line, B16 melanoma cell line, and NIH-3T3 fibroblast cell line, cultured in medium alone, were tested by flow cytometry for expression of different surface antigens. Results are presented as MFI ratios calculated as described in Materials and Methods section. B, L5178Y lymphoma cells were cultured alone or in the presence of naïve C57BL/6 Mϕ in medium, with or without LPS. After 12 hr of co-culture, non-adherent cells were harvested and tested for expression of different antigens on F4/80-negative viable L5178Y lymphoma cells by flow cytometry. The experiments are representative of three separate experiments with similar results.