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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Neuropharmacology. 2009 Jun 18;57(3):208–218. doi: 10.1016/j.neuropharm.2009.06.007

Fig 2. Prolonged exposure of epileptic cultures to WIN55,212-2 results in a concentration-dependent increase in SRED frequency and down-regulation of CB1 receptor expression.

Fig 2

Following 3 hours of low Mg2+ treatment to induce SREDs, epileptic cultures were exposed to varying concentrations (0-1000 nM) of WIN 55,212-2 (+WIN) for 24 hours and then underwent electrophysiological and immunocytochemical analysis. A. (top trace) Representative whole-cell current-clamp recording from an epileptic (no drug) control neuron displaying three independent SREDs. Prolonged exposure to +WIN at 10 nM, 100 nM and 1000 nM causes a concentration-dependent increase in freguency of SREDs. B. (top panel) Representative image of an epileptic (no drug) control neuronal culture showing CB1 receptor staining (FITC-green) throughout the neuronal processes. Prolonged exposure of epileptic cultures to +WIN results in a concentration-dependent decrease in staining for CB1receptor in neuronal processes. Nuclear staining of DNA with DAPI (blue) revealed that prolonged exposure to increasing concentrations of +WIN had no effect on neuronal culture density (scale = 100 microns).