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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Struct Biol. 2009 Jun 26;168(1):190–199. doi: 10.1016/j.jsb.2009.06.015

Figure 2.

Figure 2

The general synthetic scheme to make the immobilized DNA surface (c.f. Fig. 1A). 5' alkyne modified DNA (Alkyne-C6-5' DNA) is clicked to the azide-modified glass surface in step 1 at approximately 1% coverage, and the remaining free azides are then clicked with ethynyl phosphonic acid in step 2.