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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Struct Biol. 2009 Jun 26;168(1):190–199. doi: 10.1016/j.jsb.2009.06.015

Figure 3.

Figure 3

Estimation of click-immobilized DNA density. DNA-immobilized substrates are prepared with the click reaction for 2 hr using different concentrations of alkynyl-oligonucleotides. Cy5 labeled complementary DNA was then hybridized and its fluorescence intensity, reflecting the immobilized DNA density, was measured. Fluorescence intensities are calibrated with supported lipid bilayers displaying known amounts of DNA-lipid conjugates (blue triangles and dotted line, see text). The apparent surface density of immobilized DNA incubated with labeled alkynyl-oligonucleotides concentration is then estimated (red squares).