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. Author manuscript; available in PMC: 2010 Jan 9.
Published in final edited form as: Cell. 2009 Jan 9;136(1):62–74. doi: 10.1016/j.cell.2008.10.052

Figure 6. Sirt6 Deficiency Leads to Ectopic Expression of NF-κB Target Genes In Vivo.

Figure 6

(A) Schematic for unbiased screen for candidate transcriptional regulators of gene expression changes in Sirt6-/- tissues.

(B) Motif modules induced or repressed in Sirt6-/- tissues. Shown is the number of motifs that are induced or repressed in the indicated number of Sirt6-/- tissues. The motif module of NF-κB was induced in all Sirt6-/- tissues examined. Motifs labeled in gray were found to be both induced and repressed in all Sirt6-/- tissues in combination with different motifs. * indicates motifs previously implicated in a screen for transcription factor motifs responsible for driving gene expression changes in mammalian aging (Adler et al., 2007). See Table S1 for motif modules induced or repressed upon Sirt6 knockout.

(C) Sirt6 knockout leads to NF-κB target gene induction. Shown is gene expression analysis in wild-type (WT) or Sirt6-/- MEFs, following TNF-α treatment (20 ng/mL) for the indicated times, of genes enriched for NF-κB motifs in promoters and known NF-κB responsive genes (Hinata et al., 2003; Hinz et al., 2001). Note the increased red (induced expression above control) genes in the SIRT6-/- columns, particularly at 24 hours. Average expression of each column is shown at the top.