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. Author manuscript; available in PMC: 2010 Jul 20.
Published in final edited form as: J Comp Neurol. 2009 Jul 20;515(3):269–294. doi: 10.1002/cne.22040

Fig. 7.

Fig. 7

Neurospheres produced from human elderly SVZ are immunoreactive for neural stem cell proteins. (A) Within 2 weeks of plating on uncoated surfaces, adherent, neurosphere-like clusters of cells were observed with many fine processes and cells radiating outward. From an 84-year-old AD case; PMI 3.0 h. (B, C, D) Confocal micrographs of neurospheres immunoreactive for neural stem cell markers. Plating on PLL/laminin quickened the development and complexity of processes, as shown previously. SVZ neurospheres were expanded from a single-cell suspension for ~4.5 weeks in SFM with EGF/FGF, plated for one day on PLL/laminin to allow adherence, then fixed and immunostained for the neural stem cell proteins nestin, nucleostemin, and Musashi-1. From a 79-year-old Parkinson’s disease case who also had AD; PMI 2.5 h. Deletion of the primary antibodies resulted in no specific immunostaining. (E) Confocal micrograph of a neurosphere 2 weeks after plating, showing nestin-immunoreactive core (green) and differentiating GFAP-immunoreactive cells (red) at the periphery (same case and culture as in A). Scale bars: A, 250 μm; B, C, D, 100 μm; E, 50 μm.