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. Author manuscript; available in PMC: 2010 Jul 20.
Published in final edited form as: J Comp Neurol. 2009 Jul 20;515(3):269–294. doi: 10.1002/cne.22040

Fig. 8.

Fig. 8

Neurospheres develop only from human elderly autopsied periventricular white matter (WM) that includes the SVZ. Cultures were produced and maintained in parallel using postmortem tissue from the same case (69-year-old AD case; PMI 2.5 h) that contained either (A) periventricular WM and adjoining SVZ (cf. Fig 1A) or (B) frontal cortex and adjoining subcortical WM excluding the SVZ. Microglia were depleted as before (see Materials and Methods). Neurospheres growing on top of putative astrocytes were observed only in cultures derived from SVZ+WM; only astrocytes developed in cultures derived from neocortex+WM. Images taken 28 weeks after plating. Scale bars: A, B, 250 μm.