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. Author manuscript; available in PMC: 2009 Oct 5.
Published in final edited form as: J Mol Biol. 2007 Nov 12;375(4):1165–1177. doi: 10.1016/j.jmb.2007.11.004

Table 2. Analysis of the binding of E2 on DNA containing all 4 four BS sites.

DNA (100 ng) was incubated with 150 ng of E2 and prepared as described in Materials and Methods. ATP, KCl or DTT were included in the reaction buffer as indicated (first column). After scoring several hundred E2-DNA complexes (second column), the statistical analysis of the complexes observed is as follows: protein-free DNA (third column), DNA with a single E2 dimer (fourth column), multiple dimers on DNA (each located at a specific BS site) (fifth column), looped DNA (sixth column).

E2 on E2BS n graphic file with name nihms38816t6.jpg graphic file with name nihms38816t7.jpg graphic file with name nihms38816t8.jpg graphic file with name nihms38816t9.jpg
w/ATP 301 16% 57% 0% 27%
w/o ATP 300 48% 41% 0% 11%
w/DTT 301 7% 45% 23% 25%
w/KCI 262 38% 38% 21% 3%