FIGURE 3.

Effects of hCaMKIINβ overexpression on human ovarian cancer cell proliferation in vitro. A, morphology of HO-8910PM cells was altered by hCaMKIINβ overexpression. HO-8910PM cells were transiently transfected with hCaMKIINβ or mock control, and then cells were cultured and observed by phase contrast microscopy at the indicated time points (magnification ×10). B, proliferation of stably transfected HO-8910PM human ovarian cancer cells was examined by MTT assay. C, proliferation of stably transfected HeLa cells was examined by MTT assay. D, effect of hCaMKIINβ stable expression on the DNA synthesis of HO-8910PM cells. HO-8910PM/pKIINβ, HO-8910PM/mock, and parental HO-8910PM cells were seeded into 96-well plates and cultured for 96 h, and the proliferation of cells was detected by [³H]thymidine incorporation. E, effects of hCaMKIINβ stable expression on colony formation of HO-8910PM cells. HO-8910PM/pKIINβ, HO-8910PM/mock, and parental HO-8910PM cells (500 cells/well) were seeded into 6-well plates. Colonies were evaluated after 21 days of incubation. F, HeLa cells were transfected with si-KIINβ or mock siRNA (si-Non), and then cultured and harvested 48 h after transfection. Proliferation of cell was examined by MTT assay. *, p < 0.01 versus parental or mock cells; **, p < 0.05 versus parental or mock cells. The means and S.E. of three independent experiments are indicated.