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. 2009 Jul 15;284(37):24797–24805. doi: 10.1074/jbc.M109.022863

FIGURE 1.

FIGURE 1.

Purification of recombinant RcPPC4, AtPPC3, and a chimeric Class-2 PEPC by gel filtration FPLC. A, Superdex-200 HR 16/50 elution profile for recombinant RcPPC4. B, Superdex-200 HR 16/50 elution profile indicating the position of Class-2 and Class-1 PEPCs obtained from a mixture of recombinant RcPPC4 and AtPPC3. Fractions eluting at 46–50 and 54–59 ml were pooled to enrich Class-2 and Class-1 PEPC, respectively. C and D, Superose-6 10/300 GL elution profiles of the chimeric Class-2 PEPC (RcPPC4 + AtPPC3) hetero-octamer (C) and Class-1 PEPC (AtPPC3) homotetramer (D). Insets, aliquots (A, 7-μl each; B–D, 2-μl each) from various fractions were subjected to SDS-PAGE followed by protein staining with Coomassie Brilliant Blue R-250 (CBB-R250) (A–D), or immunoblotting with anti-RcPPC4-IgG (A). V0 denotes the void volume.