TABLE 1.

Purification of recombinant Class-1 and -2 PEPCs from combined clarified extracts originating from 3 g of AtPPC3- and 6 g of RcPPC4-expressing E. coli cells

Step	Activity	Protein	Specific activity	Purification	Yield
	units	mg	units/mg	-fold	%
Clarified extracts
AtPPC3	164	540	0.30
RcPPC4	88	1458	0.060
Combined extracts	270	2140	0.13	1	100
Ni2+-affinity FPLC	256	60	4.3	34	95
Superdex-200 FPLC
Class-1 PEPC	71.3	12.9	5.5	44	26
Class-2 PEPC	87.7	7.8	11.2	89	32
Superose-6 FPLC
Class-1 PEPC	66.6	4.4	15.1	106	25
Class-2 PEPC	60.5	2.4	25.2	152	22