FIGURE 2.

Nuclear/cytoplasmic shuttling of Foxa2 requires a functional NES. A, immunoblots showing cellular fractionation of HepG2 cells stably expressing HA-Emut Foxa2, serum-starved overnight, and stimulated with 500 nm insulin. B, immunoblots showing cellular fractionation of livers from fasted or fed C57Bl/6 mice infected with adenoviruses expressing GFP, Foxa2, T156A, or Emut. Livers of mice that were either fasted for 18 h or fed ad libitum were harvested 5 days post-infection. γ-Tubulin was used as a loading control, and LSD1 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression levels were used as the nuclear and cytosolic markers, respectively.