FIGURE 1.

AAO inhibition with p-anisic acid. pH dependence of the constant of AAO inhibition by p-anisic acid. AAO activity inhibition was measured with veratryl alcohol as substrate in the presence of p-anisic acid in air-saturated buffer at 25 °C. The inset shows the difference spectra upon p-anisic acid binding to AAO. The titration was performed at 25 °C in 100 mm phosphate, pH 6. The curves shown are the difference spectra after addition of 18, 48, 106, 278, and 698 μm p-anisic acid (in the order indicated by the arrows).