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pH dependence of the observed KIE for AAO steady state kinetic parameters. pH dependence of DVmax (A) and D(Vmax/Km) (B) for [α-2H2]p-anisyl (●) and [α-2H2]2,4-hexadien-1-ol (○) oxidation by AAO are shown. Initial rates were determined with α-protiated and α-bideuterated substrates in 100 mm (protiated) phosphate, pH 4–8.5, at 25 °C under atmospheric O2. Traces in A are the fit of data to supplemental Eq. S2.
