TABLE 1.

Kinetic parameters (of steady and pre-steady states) for AAO oxidation of p-anisyl alcohol and 2,4-hexadien-1-ol

Assays were performed in 100 mm phosphate, pH 6, at 12 °C.

	Steady statea			Pre-steady stateb
	kcat	KmS	KmO2	kred	Kd[AAO:S]
	s−1	μm	μm	s−1	μm
p-Anisyl alcohol	129 ± 5	25 ± 3	348 ± 36	139 ± 16	26 ± 5
2,4-Hexadien-1-ol	161 ± 3	91 ± 5	232 ± 13	144 ± 2	62 ± 4

^a Steady state constants were determined by varying the concentrations of both reducing substrates and O₂, and fitted to supplemental Eq. S1 that describes a ternary mechanism.

^b Kinetic traces of AAO (FAD) reduction were fitted to supplemental Eq. S6.