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. 2009 Jul 2;284(37):24840–24847. doi: 10.1074/jbc.M109.011593

TABLE 1.

Kinetic parameters (of steady and pre-steady states) for AAO oxidation of p-anisyl alcohol and 2,4-hexadien-1-ol

Assays were performed in 100 mm phosphate, pH 6, at 12 °C.

Steady statea
Pre-steady stateb
kcat KmS KmO2 kred Kd[AAO:S]
s1 μm μm s1 μm
p-Anisyl alcohol 129 ± 5 25 ± 3 348 ± 36 139 ± 16 26 ± 5
2,4-Hexadien-1-ol 161 ± 3 91 ± 5 232 ± 13 144 ± 2 62 ± 4

a Steady state constants were determined by varying the concentrations of both reducing substrates and O2, and fitted to supplemental Eq. S1 that describes a ternary mechanism.

b Kinetic traces of AAO (FAD) reduction were fitted to supplemental Eq. S6.