FIGURE 6.

Loss of Par3 or SPTLC1 expression inhibits THP-1 chemotaxis induced by structurally diverse chemokines including Fractalkine and the fMLP bacterially formylated tripeptide. A, Fractalkine (10 nm)-induced migration of THP-1 cells expressing shRNAs against Par3 or SPTLC1 is reduced by ∼80% compared with control cells expressing an shRNA targeting GFP. B, fMLP (10 nm)-induced migration of THP-1 cells expressing shRNAs against SPTLC1 or Par3 is reduced ∼60 and 80%, respectively, as compared with the control GFP shRNA cells (n = 3, ±S.D., *, p < 0.05; results are representative of two or more independent experiments).