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. 2009 Jun 25;284(37):24925–24932. doi: 10.1074/jbc.M109.026609

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Developmental and cadmium responsive bcmo-1 transcription in C. elegans. Total RNA was extracted from cadmium-treated or control C. elegans at the indicated developmental stages, and the mRNA levels of bcmo-1 were measured. (upper panel) bcmo-1 transcription at different developmental stages in C. elegans not treated with cadmium. Levels of bcmo-1 mRNA were normalized to the bcmo-1 mRNA level in L4 larvae. (lower panel) effects of 5-h exposure to 100 μm cadmium on bcmo-1 mRNA levels at different developmental stages. mRNA levels were normalized to those observed in non-exposed, control nematodes at identical developmental stages and are presented as the fold change in the levels of expression. The results are mean fold change ± standard error (n = 3); *, indicates significantly different (p < 0.05) from L4, upper panel or from untreated nematodes, lower panel. Em, embryos; Ad, adults.