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. 2009 Jun 24;284(37):24948–24957. doi: 10.1074/jbc.M109.023754

FIGURE 2.

FIGURE 2.

In vitro self-assembly of tailless NMII rod fragments. 0.1 mg/ml tailless rod fragments were expressed and purified from E. coli as described under “Experimental Procedures.” Proteins were dialyzed against different NaCl concentrations, and the extent of assembly was calculated as the percentage of rod fragment remaining in the supernatant after high speed centrifugation. Results are averages ± S.D. of at least three independent experiments.