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. 2009 Jun 24;284(37):24948–24957. doi: 10.1074/jbc.M109.023754

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — NMII-A and NMII-B tailpiece effect on isoform localization in vivo. Tailpiece-swapped full-length NMII-A and NMII-B fused to GFP were transfected into cells and subjected to wound scratch assay as described under “Experimental Procedures.” A, NMII-A tail-swapped chimeras expressed in NMII-A siRNA-treated B⁻/B⁻ MEF cells. Arrows point at cells leading edge. Red, NMII-A siRNA marker; green, GFP-NMII-A. B, NMII-B tail-swapped chimeras expressed in B⁻/B⁻ MEF cells, actin was detected by Rhodamine-Phalloidin staining. Arrows indicate posterior accumulation of NMII-B. Red, actin; green, NMII-B. Red lines are tracing of cells leading edge. Bar = 10 μm.