TABLE 2.

In vitro self-assembly of phosphomimetic forms of NMII-C rod fragment

Sedimentation assays were carried out as described in the legend to Fig. 2. NaCl concentration resulting in 50% solubility was calculated from graphs similar to those in Fig. 2. Results are averages ± S.D. of at least three independent experiments.

NMII-C Asp mutants	50% solubility
	mm NaCl ± S.D.
WT	318 ± 4.9
CKII 1973 + 1985	308 ± 3.2
CKII 1932 + 1973 + 1985	245 ± 2.3
CKII 1932 only	222 ± 8.5
PKC 1957 + 1960	185 ± 4.8
All	180 ± 3.7