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. 1998 May 26;95(11):5987–5992. doi: 10.1073/pnas.95.11.5987

Figure 5.

Figure 5

Immunoblot analysis of transgenic human SREBP-1c436 (lanes 1–4), endogenous mouse SREBP-1 (lanes 5–8), and endogenous mouse SREBP-2 (lanes 9–12) in membranes (A) and nuclear extracts (B) from livers of wild-type (WT) and TgSREBP-1c436 (Tg) mice in the nonfasted state or after fasting for 6 h as indicated. For each group, livers from mice shown in Table 2 were pooled, and aliquots (30 μg) of membranes and nuclear extracts were subjected to SDS/PAGE and electrophoretically transferred to a nitrocellulose filter. Immunoblot analysis was carried out using 5 μg/ml rabbit anti-human SREBP-1 IgG (lanes 1–4), anti-mouse SREBP-1 IgG (lanes 5–8), or anti-mouse SREBP-2 IgG (lanes 9–12) as the primary antibody. The anti-mouse SREBP-1 IgG antibody is specific for mouse SREBP-1 and does not cross-react with the human SREBP-1 transgene product. Bound antibodies were visualized as described in the text. Filters were exposed to film for 15 s for the truncated human SREBP-1, 25 s for mouse SREBP-1, and 90 s for mouse SREBP-2.